David Maddison

Carabids desired for DNA sequencing studies

Below is a list of carabid beetles I desire for our DNA sequencing studies. Some of these are for my personal projects, others for projects with my colleagues.

I realize that some of these are rare (some may be extinct), and so I don't expect to get many of them! However, please note that even a single leg should be sufficient for all but the smallest species.

Taxa marked is red are the ones of the highest priority.

To preserve specimens for DNA work, they should be placed in either 95% ethanol or dry silica gel.

Preservation in 95% ethanol: Drop the live beetles into 95% ethanol. Most important is the replacement of body fluids as quickly as possible by ethanol, and to have lots of ethanol relative to biomass. I normally try to have at least four times as much ethanol as beetle. For large specimens, or if there is a lot of beetle biomass relative to ethanol, it is important to change the ethanol a few hours after the beetles die, and then a day or two later. If the specimens are very large (>2 cm) it is ideal to crack them open at the prothorax/mesothorax joint, so that the ethanol can penetrate the thoracic muscles more quickly.

Preservation in silica gel: A vial half-filled with dry silica gel, with a cotton plug holding in the silica gel, should be prepared. One or two beetles (or more, if the beetles are very small) are placed alive between the cotton plug and the vial cap.The vial should be closed tightly. Place in the sun to dry out the specimens as quickly as possible. Keep in as dry a place as possible.

I can supply ethanol vials or silica gel vials if needed.

If you have any of these preserved in 95% ethanol or silica gel, or even in 70% ethanol, and might be able to spare a specimen or the leg of a specimen, please contact David Maddison at beetle@ag.arizona.edu.

If you would like specimens of beetles or other insects from Arizona in exchange, either in 95% ethanol, 75% ethanol, or dried, please ask.




©2006 David R. Maddison